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elisa buffer  (Chondrex Inc)


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    Structured Review

    Chondrex Inc elisa buffer
    Elisa Buffer, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 96/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+buffer/ChonBlock+Blocking%2FSample+Dilution+ELISA+Buffer/pmc13098406-51-32-34
    Average 96 stars, based on 110 article reviews
    elisa buffer - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Reimagining the serocatalytic model for infectious diseases: A case study of common coronaviruses.
    Article Snippet: Of note, the S1 subunits of spike protein (His tag) of HCoV-229E (Seattle/USA/SC1073/2016), HCoV-HKU1 (Hong Kong/isolate N5/2006), HCoV-NL63 (Florida/UF-2/2015) and the hemagglutinin-esterase (HE) protein (His Tag) of HCoV-OC43 (Seattle/USA/SC9741/2016) were purchased from Sino Biological (China). .. On the next day, the plates were blocked with 100 μl of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc, Redmon, US) and incubated at room temperature for 1 h. Each human plasma sample was diluted to 1:100 in Chonblock blocking/sample dilution ELISA buffer and then added to the ELISA plates for a 2-h incubation at 37 ◦C. .. Plates were then washed thrice and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Thermo Fisher Scientific) at 1:5000 dilution for 1 h at 37 ◦C.

    Article Title: A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection
    Article Snippet: .. For interface with lateral flow, the reaction mixture was made up to a minimum of 48 μl in volume (usually 1:4 or 1:5 dilution) with ChonBlock Blocking/Sample Dilution ELISA Buffer (Chondrex) before dipsticks (Milenia Biotec) were inserted. .. For interface with lateral flow, the reaction mixture was made up to a minimum of 48 μl in volume (usually 1:4 or 1:5 dilution) with ChonBlock Blocking/Sample Dilution ELISA Buffer (Chondrex) before dipsticks (Milenia Biotec) were inserted.

    Article Title: Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity
    Article Snippet: .. The plates were then blocked with 100 μL of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc., Redmond, US) and incubated at room temperature for 1 h. For human plasma samples, each sample was diluted to an initial concentration of 1:100, followed by ten-fold dilutions (1:1000, 1:10,000, 1:100,000) prepared in Chonblock blocking/sample dilution ELISA buffer and added to the antigen wells. ..

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine encoding African swine fever virus p54 antigen elicits robust immune responses in pigs
    Article Snippet: .. After fixation with an acetone/methanol mixture, the cells were incubated for 1 h with swine serum samples, which were diluted 1:10 in ChonBlock blocking/sample dilution ELISA buffer (Chondrex Inc., WA, USA). ..

    Article Title: Four doses of coronavirus disease 2019 vaccination for patients with inborn errors of immunity compared to 3 doses for healthy individuals
    Article Snippet: .. In brief, S-RBD IgG ELISA plates were coated overnight with 100 ng per well of purified recombinant S-RBD in phosphate-buffered saline (PBS), followed by the addition of 100 μL Chonblock Blocking/Sample Dilution ELISA buffer (Chondrex, Redmond, Wash). ..

    Article Title: Differential antigenic imprinting effects between influenza H1N1 hemagglutinin and neuraminidase in a mouse model
    Article Snippet: Nunc MaxiSorp ELISA plates (Thermo Fisher Scientific) were coated overnight at 4°C with 100 μL of recombinant proteins at 1 μg/mL in 1 × PBS. .. On the next day, plates were washed three times with 1 × PBS containing 0.05% Tween 20 and blocked with 100 μL of ChonBlock blocking/sample dilution ELISA buffer (Chondrex Inc, Redmond, US) for 1 hour at room temperature. ..

    Article Title: Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity
    Article Snippet: .. The plates were then blocked with 100 μL of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc., Redmond, US) and incubated at room temperature for 1 hour. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Reimagining the serocatalytic model for infectious diseases: A case study of common coronaviruses.
    Article Snippet: Of note, the S1 subunits of spike protein (His tag) of HCoV-229E (Seattle/USA/SC1073/2016), HCoV-HKU1 (Hong Kong/isolate N5/2006), HCoV-NL63 (Florida/UF-2/2015) and the hemagglutinin-esterase (HE) protein (His Tag) of HCoV-OC43 (Seattle/USA/SC9741/2016) were purchased from Sino Biological (China). .. On the next day, the plates were blocked with 100 μl of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc, Redmon, US) and incubated at room temperature for 1 h. Each human plasma sample was diluted to 1:100 in Chonblock blocking/sample dilution ELISA buffer and then added to the ELISA plates for a 2-h incubation at 37 ◦C. .. Plates were then washed thrice and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Thermo Fisher Scientific) at 1:5000 dilution for 1 h at 37 ◦C.

    Article Title: A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection
    Article Snippet: .. For interface with lateral flow, the reaction mixture was made up to a minimum of 48 μl in volume (usually 1:4 or 1:5 dilution) with ChonBlock Blocking/Sample Dilution ELISA Buffer (Chondrex) before dipsticks (Milenia Biotec) were inserted. .. For interface with lateral flow, the reaction mixture was made up to a minimum of 48 μl in volume (usually 1:4 or 1:5 dilution) with ChonBlock Blocking/Sample Dilution ELISA Buffer (Chondrex) before dipsticks (Milenia Biotec) were inserted.

    Article Title: Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity
    Article Snippet: .. The plates were then blocked with 100 μL of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc., Redmond, US) and incubated at room temperature for 1 h. For human plasma samples, each sample was diluted to an initial concentration of 1:100, followed by ten-fold dilutions (1:1000, 1:10,000, 1:100,000) prepared in Chonblock blocking/sample dilution ELISA buffer and added to the antigen wells. ..

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine encoding African swine fever virus p54 antigen elicits robust immune responses in pigs
    Article Snippet: .. After fixation with an acetone/methanol mixture, the cells were incubated for 1 h with swine serum samples, which were diluted 1:10 in ChonBlock blocking/sample dilution ELISA buffer (Chondrex Inc., WA, USA). ..

    Article Title: Four doses of coronavirus disease 2019 vaccination for patients with inborn errors of immunity compared to 3 doses for healthy individuals
    Article Snippet: .. In brief, S-RBD IgG ELISA plates were coated overnight with 100 ng per well of purified recombinant S-RBD in phosphate-buffered saline (PBS), followed by the addition of 100 μL Chonblock Blocking/Sample Dilution ELISA buffer (Chondrex, Redmond, Wash). ..

    Article Title: Differential antigenic imprinting effects between influenza H1N1 hemagglutinin and neuraminidase in a mouse model
    Article Snippet: Nunc MaxiSorp ELISA plates (Thermo Fisher Scientific) were coated overnight at 4°C with 100 μL of recombinant proteins at 1 μg/mL in 1 × PBS. .. On the next day, plates were washed three times with 1 × PBS containing 0.05% Tween 20 and blocked with 100 μL of ChonBlock blocking/sample dilution ELISA buffer (Chondrex Inc, Redmond, US) for 1 hour at room temperature. ..

    Article Title: Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity
    Article Snippet: .. The plates were then blocked with 100 μL of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc., Redmond, US) and incubated at room temperature for 1 hour. ..

    Incubation:

    Article Title: Reimagining the serocatalytic model for infectious diseases: A case study of common coronaviruses.
    Article Snippet: Of note, the S1 subunits of spike protein (His tag) of HCoV-229E (Seattle/USA/SC1073/2016), HCoV-HKU1 (Hong Kong/isolate N5/2006), HCoV-NL63 (Florida/UF-2/2015) and the hemagglutinin-esterase (HE) protein (His Tag) of HCoV-OC43 (Seattle/USA/SC9741/2016) were purchased from Sino Biological (China). .. On the next day, the plates were blocked with 100 μl of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc, Redmon, US) and incubated at room temperature for 1 h. Each human plasma sample was diluted to 1:100 in Chonblock blocking/sample dilution ELISA buffer and then added to the ELISA plates for a 2-h incubation at 37 ◦C. .. Plates were then washed thrice and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Thermo Fisher Scientific) at 1:5000 dilution for 1 h at 37 ◦C.

    Article Title: Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity
    Article Snippet: .. The plates were then blocked with 100 μL of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc., Redmond, US) and incubated at room temperature for 1 h. For human plasma samples, each sample was diluted to an initial concentration of 1:100, followed by ten-fold dilutions (1:1000, 1:10,000, 1:100,000) prepared in Chonblock blocking/sample dilution ELISA buffer and added to the antigen wells. ..

    Article Title: Lipid nanoparticle-encapsulated DNA vaccine encoding African swine fever virus p54 antigen elicits robust immune responses in pigs
    Article Snippet: .. After fixation with an acetone/methanol mixture, the cells were incubated for 1 h with swine serum samples, which were diluted 1:10 in ChonBlock blocking/sample dilution ELISA buffer (Chondrex Inc., WA, USA). ..

    Article Title: Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity
    Article Snippet: .. The plates were then blocked with 100 μL of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc., Redmond, US) and incubated at room temperature for 1 hour. ..

    Clinical Proteomics:

    Article Title: Reimagining the serocatalytic model for infectious diseases: A case study of common coronaviruses.
    Article Snippet: Of note, the S1 subunits of spike protein (His tag) of HCoV-229E (Seattle/USA/SC1073/2016), HCoV-HKU1 (Hong Kong/isolate N5/2006), HCoV-NL63 (Florida/UF-2/2015) and the hemagglutinin-esterase (HE) protein (His Tag) of HCoV-OC43 (Seattle/USA/SC9741/2016) were purchased from Sino Biological (China). .. On the next day, the plates were blocked with 100 μl of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc, Redmon, US) and incubated at room temperature for 1 h. Each human plasma sample was diluted to 1:100 in Chonblock blocking/sample dilution ELISA buffer and then added to the ELISA plates for a 2-h incubation at 37 ◦C. .. Plates were then washed thrice and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Thermo Fisher Scientific) at 1:5000 dilution for 1 h at 37 ◦C.

    Article Title: Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity
    Article Snippet: .. The plates were then blocked with 100 μL of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc., Redmond, US) and incubated at room temperature for 1 h. For human plasma samples, each sample was diluted to an initial concentration of 1:100, followed by ten-fold dilutions (1:1000, 1:10,000, 1:100,000) prepared in Chonblock blocking/sample dilution ELISA buffer and added to the antigen wells. ..

    Concentration Assay:

    Article Title: Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity
    Article Snippet: .. The plates were then blocked with 100 μL of Chonblock blocking/sample dilution ELISA buffer (Chondrex Inc., Redmond, US) and incubated at room temperature for 1 h. For human plasma samples, each sample was diluted to an initial concentration of 1:100, followed by ten-fold dilutions (1:1000, 1:10,000, 1:100,000) prepared in Chonblock blocking/sample dilution ELISA buffer and added to the antigen wells. ..

    other:

    Article Title: TRIM21 is a molecular rheostat for influenza A virus replication
    Article Snippet: Cells were then treated overnight with 2000 U/mL of universal Type I IFN (PBL Bioscience) and lysed at 24 hours post treatment using Passive Lysis Buffer (Promega).

    Purification:

    Article Title: Four doses of coronavirus disease 2019 vaccination for patients with inborn errors of immunity compared to 3 doses for healthy individuals
    Article Snippet: .. In brief, S-RBD IgG ELISA plates were coated overnight with 100 ng per well of purified recombinant S-RBD in phosphate-buffered saline (PBS), followed by the addition of 100 μL Chonblock Blocking/Sample Dilution ELISA buffer (Chondrex, Redmond, Wash). ..

    Recombinant:

    Article Title: Four doses of coronavirus disease 2019 vaccination for patients with inborn errors of immunity compared to 3 doses for healthy individuals
    Article Snippet: .. In brief, S-RBD IgG ELISA plates were coated overnight with 100 ng per well of purified recombinant S-RBD in phosphate-buffered saline (PBS), followed by the addition of 100 μL Chonblock Blocking/Sample Dilution ELISA buffer (Chondrex, Redmond, Wash). ..

    Saline:

    Article Title: Four doses of coronavirus disease 2019 vaccination for patients with inborn errors of immunity compared to 3 doses for healthy individuals
    Article Snippet: .. In brief, S-RBD IgG ELISA plates were coated overnight with 100 ng per well of purified recombinant S-RBD in phosphate-buffered saline (PBS), followed by the addition of 100 μL Chonblock Blocking/Sample Dilution ELISA buffer (Chondrex, Redmond, Wash). ..



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